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It has been postulated that increased energy expenditure results in NYU student Chelsea Clinton Bernie 2020 Shirt shortened survival. To test this “rate-of-living theory” we examined the effect of raising energy expenditure by means of cold exposure on the longevity of rats. Male rats were gradually accustomed to immersion in cool water. After 3 mo they were standing in the cool water for 4 h/day, 5 days/wk. They were maintained on this program until age 32 mo. The cold exposure resulted in an increase in food intake.
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Despite their greater food intake, the cold-exposed rats’ body weights were significantly lower than those of control animals from age to mo. The average age at death of the cold-exposed rats was days compared with days for the controls. The cold exposure appeared to protect against neoplasia, particularly sarcomas; only of the necropsied cold-exposed rats had malignancies compared with for the controls. The results of this study provide no support for the concept that increased energy expenditure decreases longevity.
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Identifying structural limitations in transport is primarily restricted by current methods employed to characterize the nature of physiological remodeling. Inadequate resolution or breadth of available data has impaired the development of routine diagnostic protocols and effective therapeutic strategies. Understanding transport within striated muscle faces major challenges most notably in quantifying how well individual fibers are supplied by the microcirculation, which has necessitated exploring tissue supply using theoretical modeling of diffusive exchange.
With capillary domains identified as a suitable model for the description of local supply and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high-throughput method for histological analysis. We present an NYU student Chelsea Clinton Bernie 2020 Shirt integrated package that identifies optimal protocols for identification of important input elements, processing of digitized images with semiautomated routines.