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These studies address issues of fiber architecture and Luke Perry Death 90210 Shirt distribution of neuromuscular junctions on the surfaces of the diaphragm. From these, we inferred the mechanism of tension transmission between muscle fibers and connective tissue. Using morphological techniques, we examined the fiber architecture, including determining the number of fibers per cross-sectional unit area and the ratio of connective tissue to muscle fibers, as well as the lengths of four well-identified muscle fascicles and the lengths of the multiple NMJ bands from the mid-coastal region of the left hemidiaphragm from one dog.
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Fiber cross-sectional shape, and fiber cross-sectional area along the course of muscle from to. Using histochemical methods, we examined the NMJ distribution on the thoracic and abdominal surfaces of the costal and rural regions of the diaphragm. Both morphological and histochemical studies supported the hypothesis of a discontinuous fiber architecture in which tension generated by active muscle fibers in the diaphragm is transmitted not only along fibers spanning from to but also among muscle fibers through the connective tissue surrounding those fibers. To illustrate the methods in simple form, we show in Table.
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Care was taken to cut the samples, cm wide, cm from the area where the muscles were pinned. Samples taken were placed in histological cassettes, cut into transverse sections, and stained by using a trichrome staining technique. This technique allowed intracellular structures of different architecture and composition to be visibly varied: the muscle fibers and connective tissue of our samples stained red and blue, respectively. For each section, 20 fields were analyzed according to the following protocol. The longest fascicles of the mid-coastal region were crossed by a series of four or five short bands spaced measuring major fiber diameter.
Our measurements were started at the top right-hand corner of each section and moved horizontally to analyze every fourth field. We skipped every other row and stayed at least one field of vision away from the boundaries of the section. Within each field, the number of points falling on muscle fibers, connective tissue, and other tissue material was counted. From these data, we were able to compute an estimate of the fractional content of connective tissue vs. muscle fiber, the percentage of Luke Perry Death 90210 Shirt tissue components.