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Before sampling at the embryo stage of a hole was made in all individuals to Dory I’m Never Drinking Again Oh Look Whiskey Shirt based on the method of Moore with slight modifications. All embryos at this stage were injected with Before injection, the eggs were candled to determine the location of the abdomen, and a mark was placed on the egg to indicate the site of the entrance. Once the site of the entrance was determined, the egg was washed with 75% ethanol. A small hole of 8 mm in diameter was incised in the eggshell directly above the site of the intended injection.
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Once injected, the holes in the eggshell were sealed with cellophane tape, and the eggs were returned to the incubator until sampling. After BrdU injection, was allowed for them to be incorporated into the nuclei entering the of the cell cycle. After the time had elapsed, all of the breast and leg muscles were isolated for sampling. Breast and leg muscles were isolated from embryos from each group and stage. All samples were separated and weighted, and samples from five individuals from each treatment and stage were immediately frozen in liquid nitrogen and then stored at extraction.
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Samples from another 10 individuals from both treatments at each stage were dipped in sucrose for at room temperature and then stored at a freezer for frozen sections and morphological analysis. Samples from other individuals were kept in formalin for paraffin sections fabrication. All procedures of our experiment were approved by the Beijing Animal Welfare Committee. In the future, more precise methods should be studied to yield convincing data regarding myoblast proliferation during embryonic development in poultry.
Moore et al. established this methodology for researching muscle cell proliferation in the late-term embryonic poultry. However, many other types of cells exist in the embryonic phase in addition to myoblasts and myofibers and are incorporated into all newly, therefore, it is commonly used as a marker to Dory I’m Never Drinking Again Oh Look Whiskey Shirt identify all replicating nuclei, which includes all types of muscle cells. In this study, the muscle nuclei were distinct from other tissues due to their locations in muscle fibers.
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