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Fixed tissues were washed with running Deadpool I Am Currently Unsupervised I Know It Freaks Me Out Too But The Possibilities Are Endless Shirt water and then dehydrated in a series of ethanol dilutions for overnight, and then with two changes. Dehydrated tissues were treated with xylene three times and then embedded into paraffin blocks, trimmed, and cut to. A paraffin ribbon was placed in a water bath at about. Sections were mounted onto slides, air dried for 30 min, and then baked at overnight. Sections were dewaxed with two changes of xylene for 10 min each and then hydrated in two changes of ethanol for 1 min each.
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And, finally, rinsed in distilled water for 5 min. Slices were stained with hematoxylin-eosin. All sections were photographed using a digital microscope. Five individuals were analyzed per group; five sections were studied per individual by monitoring five random fields per section. The total average of myofibers was examined per bird studied. The myofiber diameter and the mean numbers of myofibers in the unit area were measured by software. It then increases for a few seconds before it stabilizes at a level similar to that recorded when the duck is swimming fairly vigorously.
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The present study has shown that, following bilateral denervation of the carotid bodies, there is a significant increase in mean dive duration but no effect on the immediate reduction in heart rate on submersion. Heart rate is, however, significantly higher toward the end of spontaneous dives after carotid body denervation. Unlike the situation in mallards and their domesticated varieties, carotid body denervation has no effect on heart rate in tufted ducks during the first 40 s of forced dives.
The carotid bodies, therefore, do not play the dominant role in cardiac control during submersion of diving ducks that has been suggested by work involving the forcible submersion of the mallard duck and its domesticated varieties. Experimental perfusion of the brain and single intact carotid body in unanesthetized ducks with hyperoxic blood during low Deadpool I Am Currently Unsupervised I Know It Freaks Me Out Too But The Possibilities Are Endless Shirt breathing resulted in tachypnea, also after a considerable latency. These results suggest that severe hypoxia can affect breathing in birds via pathways other than those involving the carotid bodies.